By WDQ OPTICS | 16 September 2021 | 0 Comments
How To Choose Optical Fluorescent Filter?
How To Choose Optical Fluorescent Filter?
Application fields of fluorescent filter:
Fluorescence microscope, high-performance multi-channel real-time fluorescence quantitative PCR instrument, high-grade flow cytometry analyzer, DNA technology analyzer Biochemical, immune, and molecular diagnostic instruments such as blood cell analyzer and hemoglobin detector, and quantitative fluorescence measurement in tissues and cells Determination, cell membrane analysis, single-molecule structure analysis, fluorescent living cell imaging, time-delay imaging, microstructure and single-molecule imaging,Cell membrane_ Single protein dynamics, calcium detection, drug tracking and cell structure imaging.
How to choose a fluorescent filter?
The selection principle of fluorescence filter is to let the fluorescence/emission light pass through as much as possible at the imaging end, and completely block the excitation light to obtain the highest signal-to-noise ratio. Especially for the application of multiphoton excitation and total internal reflection microscopy, weak noise will also have an effect on the imaging, As a result, it causes great interference, so the requirement for a signal-to-noise ratio is higher.
Usually, we look at the parameters of the filter and generally look at the transmittance spectrum, but the intensity of fluorescence relative to excitation is very weak, so we choose the filter needs to refer to the blocking depth spectrum - OD value. 0d value, which can more directly reflect the blocking characteristics of the filter to light.
When selecting excitation filter and emission filter, the spectral intersection of blocking depth (0d intersection) is an important criterion
Generally, the excitation of the wide-field light source is the intersection σ The D value is greater than 5 and greater than 0d6 when excited by laser, so as to achieve a better fluorescence signal Noise ratio.
Application fields of fluorescent filter:
Fluorescence microscope, high-performance multi-channel real-time fluorescence quantitative PCR instrument, high-grade flow cytometry analyzer, DNA technology analyzer Biochemical, immune, and molecular diagnostic instruments such as blood cell analyzer and hemoglobin detector, and quantitative fluorescence measurement in tissues and cells Determination, cell membrane analysis, single-molecule structure analysis, fluorescent living cell imaging, time-delay imaging, microstructure and single-molecule imaging,Cell membrane_ Single protein dynamics, calcium detection, drug tracking and cell structure imaging.
How to choose a fluorescent filter?
The selection principle of fluorescence filter is to let the fluorescence/emission light pass through as much as possible at the imaging end, and completely block the excitation light to obtain the highest signal-to-noise ratio. Especially for the application of multiphoton excitation and total internal reflection microscopy, weak noise will also have an effect on the imaging, As a result, it causes great interference, so the requirement for a signal-to-noise ratio is higher.
Usually, we look at the parameters of the filter and generally look at the transmittance spectrum, but the intensity of fluorescence relative to excitation is very weak, so we choose the filter needs to refer to the blocking depth spectrum - OD value. 0d value, which can more directly reflect the blocking characteristics of the filter to light.
When selecting excitation filter and emission filter, the spectral intersection of blocking depth (0d intersection) is an important criterion
Generally, the excitation of the wide-field light source is the intersection σ The D value is greater than 5 and greater than 0d6 when excited by laser, so as to achieve a better fluorescence signal Noise ratio.
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